The relatively recent appreciation of stem cells in native tissue has stimulated research to garner them into chondrogenic replacements. This study compared cartilage, synovium, and adipose tissue as sources for osteochondral repair. Tissue was harvested from the grossly normal stifle joints from 6 dogs, expanded in monolayer culture, then exposed to osteogenic and chondrogenic media. Gene expression and biochemical, histologic, and immunohistochemical analyses were performed to identify osteogenic and chondrogenic potential. Cartilage cells were a superior source in their chondrogenic properties (eg, larger volume, proteoglycan-rich extracellular matrix, collagen mRNA expression). Adipose tissue and synovium were similarly poor in all evaluated measures. No tissue sources provided consistent evidence of osteogenic potential.