This study compared the benefits of two hemostatic agents in postoperative hepatic bleeding. Because of the liver’s high vascularity, biopsy, trauma, or resection can produce bleeding that may be difficult to control. This study compared a thrombin-containing new-generation topical surgical hemostatic agent (gelatin matrix) to a hemostatic gelatin sponge. To construct the matrix, a bovine-derived gelatin matrix and a human-derived thrombin component are combined in the surgical field to create 5 mL of a highly viscous, flowable gel that can be applied to the surgery site of the liver using a syringe and plastic applicator tip. Each of 14 dogs had 2 liver biopsies taken via laparotomy. One biopsy site was treated with the gelatin matrix, the other with a gelatin sponge. Sites treated with the gelatin matrix achieved hemostasis more quickly (136 seconds vs 373 seconds) and had less blood loss (0.12 mL vs 1.18 mL). Adverse reactions were monitored for the first 24 hours; none were seen. The gelatin matrix was concluded to be a safe and more effective means to control bleeding after taking biopsies from the liver.