The canine pancreatic lipase immunoreactivity (cPLI) assay has significant utility in clinical practice as an adjunct diagnostic test for canine pancreatitis. This study assessed the stability of cPLI in serum and plasma samples at different temperatures and after prednisone administration in canine samples. Clinical canine serum samples (n = 8) were stored at various temperatures and were assayed at days 0, 3, 7, 14, and 21. In addition, 30 time-matched serum and plasma samples were examined; each pair was drawn from a single dog. Healthy adult female dogs with X-linked hereditary nephritis syndrome undergoing prednisone therapy were included as well. The cPLI enzyme-linked immunosorbent assay was performed on all samples. Median serum and plasma cPLI concentrations were strongly correlated, and these paired values did not significantly differ from one another. Mean serum cPLI concentrations did not vary significantly in samples stored at different temperatures on different days from day 0 to day 21. The greatest variation was noted in samples with the lowest and highest concentrations. Mean serum cPLI measurements did not exhibit significantly different pre- and postprednisone measurements and did not change significantly over time. Postprednisone serum cPLI concentrations were within reference ranges for all dogs at all time points. Therefore, cPLI measured in canine serum samples stored in a refrigerator, at room temperature, or in a freezer remained stable for at least 21 days. In addition, prednisone therapy did not appear to influence or alter basal cPLI concentrations.
COMMENTARY: This study supports the robustness of cPLI measurements in the diagnosis of canine pancreatitis. It suggests that the assay can accurately withstand a variety of temperatures, sample types, and clinical considerations. Plasma can be used for cPLI measurements in clinical situations where serum is difficult to obtain. Finally, cPLI does not appear to be influenced by steroid therapy, which has widespread use in veterinary medicine. This study indicates that cPLI is an excellent and robust diagnostic modality in dogs with pancreatitis. —Indu Mani, DVM, DSc (infectious disease)
Stability of canine pancreatic lipase immunoreactivity concentration in serum samples and effects of long-term administration of prednisone to dogs on serum canine pancreatic lipase immunoreactivity concentrations. Steiner JM, Teague SR, Lees GE, et al. AM J VET RES 70:1001-1005, 2009.