Changes in plasma proteins caused by obesity-related factors can be connected to the development of metabolic disorders. Proteome analysis (ie, studying global protein expression) has been performed in studies of human and experimental animal models to clarify the physiopathology of obesity and its associated diseases.

This study investigated how a weight-loss program may alter the proteome of canine serum. Five adult, female, obese beagles were included; all began the study >30% overweight and remained in the study for 3 months until a BCS compatible with ideal body weight was attained. The dogs were fed a restricted diet of hypoenergetic commercial food q24h. The amount of food was progressively adjusted to induce rapid weight loss of 2%–3% per week. Protein profiles were obtained, and before (ie, obese) and after (ie, lean) samples were compared, giving a total of 144 spot matches. Three of the spot matches had modulated expressions in response to weight loss. Two protein spots were upregulated and 1 protein spot downregulated in the obese state compared with the lean state. These protein spots corresponded to retinol-binding protein 4, clusterin precursor, and α1-antitrypsin. These may be potential markers of obesity and obesity-related disease processes in dogs.

A proteomic analysis of serum from dogs before and after a controlled weight-loss program. Tvarijonaviciute A, Gutiérrez AM, Miller I, et al. DOM ANIM ENDOCRINOL 43:271-277, 2012.