Described techniques for liver biopsy in dogs include fine-needle aspiration, ultrasound-guided percutaneous core needle biopsy, celiotomy, and minimally invasive laparoscopic liver biopsy (LLB). LLB combines the benefits of minimally invasive surgery with the ability to grossly visualize the liver and collect larger samples in an optimal location. There are potential drawbacks to LLB, including technical difficulty and potential for uncontrollable hemorrhage, necessitating conversion to open laparotomy. The rate of these complications, however, is unknown. The records of 106 client-owned dogs that underwent LLB were reviewed retrospectively to determine the complication and conversion rates associated with single or multiport LLB in 2 veterinary teaching hospitals. Of the 94 dogs with coagulation profiles present in the record, 25/94 (26.6%) had a coagulopathy prior to surgery. Of these, 84 had automated platelet counts performed (21/84, or 25%, were thrombocytopenic) and 10 had adequate platelet numbers on manual blood smear. Fourteen dogs, 10 of which were coagulopathic, received fresh frozen plasma before surgery. The multiport technique was used in 99/106 dogs, and the single port technique was used in 7/106 dogs. All patients had a sample of diagnostic quality obtained. Splenic laceration during port placement required conversion to an open laparotomy in 2/106 dogs. No other intraoperative complications were recorded. Five dogs were euthanized before discharge for reasons unrelated to the procedure. The authors concluded that both single and multi-port LLB are safe, effective, minimally invasive diagnostic techniques with low rates of complications.