In 2016, a novel protoparvovirus similar to human bufaviruses was identified in dogs with respiratory signs and termed canine bufavirus. Considering the ability of canine parvovirus type 2 variants to infect and cause clinical signs in cats, the authors of this study investigated whether this new canine bufavirus could do the same. A total of 574 archival feline nasal and oropharyngeal swabs and enteric samples from 2 universities in Italy were analyzed. Bufavirus DNA was identified in 9.2% of samples. DNA was most commonly identified in samples from the respiratory tract. The role of this novel virus in feline respiratory disease complex warrants further investigation.