Honey has been used to treat a variety of wounds. The antibacterial effects of honey have been partly attributed to its hydrogen peroxide (H2O2) activity, but catalases present in tissue and chronic wounds can potentially render H2O2 inactive. In this study,* cultures of Malassezia pachyderamatis and methicillin-susceptible and methicillin-resistant Staphylococcus pseudintermedius—important components of otitis externa and canine pyoderma, respectively—were subjected to serial dilutions of a honey-based gel or honey alone. Synthesized honey, triclosan, and clotrimazole served as controls. Higher antibacterial activity of the honey-based gel as compared with honey alone was noted; further studies documenting in vivo effectiveness in the treatment of clinical canine pyoderma and otitis externa cases are needed.