Leptospirosis is an important disease differential in dogs with systemic illness, but testing for this potentially fatal, zoonotic disease is difficult, as no test is consistently 100% sensitive (ie, no false-negative results) or 100% specific (ie, no false-positive results).
Diagnostic laboratories offer quantitative tests for leptospiral antibodies (serovar titers by microscopic agglutination testing [MAT]) and antigens (conventional PCR or real-time quantitative PCR [qPCR] tests of blood and urine) for disease diagnosis. More recently, rapid, in-house, point-of-care immunodiagnostic tests for detection of immunoglobulin M and/or immunoglobulin G have become available to clinicians.1,2
This study evaluated and compared 2 in-house tests for diagnosis of acute leptospirosis in dogs. Dogs with suspected acute leptospirosis (n = 89) were tested, and results were compared with a clinical diagnosis based on MAT and/or qPCR. Classification of 42 dogs with confirmed leptospirosis was based on a single high MAT titer on admission (n = 32), a 4-fold increase in convalescent MAT (n = 4) results, and/or a positive blood (n = 5) and/or urine qPCR (n = 5) result. These results suggest differing duration of infection in these clinical cases.
Evaluation of the point-of-care antibody tests compared with this classification indicated both tests to be comparable with and possibly more sensitive than MAT. Comparison of calculated sensitivity, specificity, and accuracy is difficult due to the variety of clinical cases, duration of illness, and criteria used to classify cases as confirmed. Nevertheless, in-house tests that detect immunoglobulin M may signal increased titers a few days earlier than immunoglobulin G-based tests.3