Microbiota Modulation Techniques in Dogs

AUTHOR

Jan S. Suchodolski

MedVet, DrVetMed, PhD, AGAF, DACVM (Immunology) Gastrointestinal Laboratory at Texas A&M University

Jan S. Suchodolski, MedVet, DrVetMed, PhD, AGAF, DACVM (Immunology), is an associate professor in small animal medicine, the associate director for research, and the head of microbiome sciences at the Gastrointestinal Laboratory at Texas A&M University, where he earned his PhD in veterinary microbiology. Dr. Suchodolski is a graduate of University of Vienna in Vienna, Austria. He has authored or coauthored more than 340 peer-reviewed articles on veterinary gastroenterology and microbiome research. His research is focused on developing biomarkers for GI disease and therapeutic approaches for modulation of the intestinal microbiota.

Benyacoub J, Czarnecki-Maulden GL, Cavadini C, et al. Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. J Nutr. 2003;133(4):1158-1162.
Rossi G, Pengo G, Galosi L, et al. Effects of the probiotic mixture Slab51 (SivoMixx) as food supplement in healthy dogs: evaluation of fecal microbiota, clinical parameters and immune function. Front Vet Sci. 2020;7:613.
Bybee SN, Scorza AV, Lappin MR. Effect of the probiotic Enterococcus faecium SF68 on presence of diarrhea in cats and dogs housed in an animal shelter. J Vet Intern Med. 2011;25(4):856-860.
Ziese AL, Suchodolski JS, Hartmann K, et al. Effect of probiotic treatment on the clinical course, intestinal microbiome, and toxigenic Clostridium perfringens in dogs with acute hemorrhagic diarrhea. PLoS One. 2018;13(9):e0204691.
Flickinger EA, Schreijen E, Patil AR, et al. Nutrient digestibilities, microbial populations, and protein catabolites as affected by fructan supplementation of dog diets. J Anim Sci. 2003;81(8):2008-2018.
Weese JS, Martin H. Assessment of commercial probiotic bacterial contents and label accuracy. Can Vet J. 2011;52(1):43-46.
Duboux S, Van Wijchen M, Kleerebezem M. The possible link between manufacturing and probiotic efficacy; a molecular point of view on Bifidobacterium. Front Microbiol. 2021;12:812536.
Biagioli M, Laghi L, Carino A, et al. Metabolic variability of a multispecies probiotic preparation impacts on the anti-inflammatory activity. Front Pharmacol. 2017;8:505.
Li Q, Larouche-Lebel E, Loughran KA, Huh TP, Suchodolski JS, Oyama MA. Gut dysbiosis and its associations with gut microbiota-derived metabolites in dogs with myxomatous mitral valve disease. mSystems. 2021;6(2):e00111-e00121.
AlShawaqfeh MK, Wajid B, Minamoto Y, et al. A dysbiosis index to assess microbial changes in fecal samples of dogs with chronic inflammatory enteropathy. FEMS Microbiol Ecol. 2017;93(11).
Blake AB, Cigarroa A, Klein HL, et al. Developmental stages in microbiota, bile acids, and clostridial species in healthy puppies. J Vet Intern Med. 2020;34(6):2345-2356.
Pilla R, Gaschen FP, Barr JW, et al. Effects of metronidazole on the fecal microbiome and metabolome in healthy dogs. J Vet Intern Med. 2020;34(5):1853-1866.
Li Q, Larouche-Lebel É, Loughran KA, Huh TP, Suchodolski JS, Oyama MA. Gut dysbiosis and its associations with gut microbiota-derived metabolites in dogs with myxomatous mitral valve disease. mSystems. 2021;6(2):e00111-00121.
Werner M, Suchodolski JS, Lidbury JA, Steiner JM, Hartmann K, Unterer S. Diagnostic value of fecal cultures in dogs with chronic diarrhea. J Vet Intern Med. 2021;35(1):199-208.
Manchester AC, Webb CB, Blake AB, et al. Long-term impact of tylosin on fecal microbiota and fecal bile acids of healthy dogs. J Vet Intern Med. 2019;33(6):2605-2617.
Chaitman J, Ziese AL, Pilla R, et al. Fecal microbial and metabolic profiles in dogs with acute diarrhea receiving either fecal microbiota transplantation or oral metronidazole. Front Vet Sci. 2020;7:192.
Torres-Henderson C, Summers S, Suchodolski J, Lappin MR. Effect of Enterococcus faecium strain SF68 on gastrointestinal signs and fecal microbiome in cats administered amoxicillin-clavulanate. Top Companion Anim Med. 2017;32(3):104-108.
Stokes JE, Price JM, Whittemore JC. Randomized, controlled, crossover trial of prevention of clindamycin-induced gastrointestinal signs using a synbiotic in healthy research cats. J Vet Intern Med. 2017;31(5):1406-1413.
Aktas MS, Borku MK, Ozkanlar Y. Efficacy of Saccharomyces boulardii as a probiotic in dogs with lincomycin induced diarrhoea. Bull Vet Inst Pulawy. 2007;51:365-369.
Fenimore A, Martin L, Lappin MR. Evaluation of metronidazole with and without Enterococcus faecium SF68 in shelter dogs with diarrhea. Top Companion Anim Med. 2017;32(3):100-103.
Leib MS. Treatment of chronic idiopathic large-bowel diarrhea in dogs with a highly digestible diet and soluble fiber: a retrospective review of 37 cases. J Vet Intern Med. 2000;14(1):27-32.
Procoli F. Inflammatory bowel disease, food-responsive, antibiotic-responsive diarrhoea, protein losing enteropathy. Adv Small Anim Care. 2020;1:127-141.
Volkmann M, Steiner JM, Fosgate GT, Zentek J, Hartmann S, Kohn B. Chronic diarrhea in dogs - retrospective study in 136 cases. J Vet Intern Med. 2017;31(4):1043-1055.
Allenspach K, Culverwell C, Chan D. Long-term outcome in dogs with chronic enteropathies: 203 cases. Vet Rec. 2016;178(15):368.
Bresciani F, Minamoto Y, Suchodolski JS, et al. Effect of an extruded animal protein-free diet on fecal microbiota of dogs with food-responsive enteropathy. J Vet Intern Med. 2018;32(6):1903-1910.
Wang S, Martins R, Sullivan MC, et al. Diet-induced remission in chronic enteropathy is associated with altered microbial community structure and synthesis of secondary bile acids. Microbiome. 2019;7(1):126.
Schmitz SS. Value of probiotics in canine and feline gastroenterobiology. Vet Clin North Am Small Anim Pract. 2021;51(1):171-217.
White R, Atherly T, Guard B, et al. Randomized, controlled trial evaluating the effect of multi-strain probiotic on the mucosal microbiota in canine idiopathic inflammatory bowel disease. Gut Microbes. 2017;8(5):451-466.
D'Angelo S, Fracassi F, Bresciani F, et al. Effect of Saccharomyces boulardii in dog with chronic enteropathies: double-blinded, placebo-controlled study. Vet Rec. 2018;182(9):258.
Weingarden AR, Chen C, Bobr A, et al. Microbiota transplantation restores normal fecal bile acid composition in recurrent Clostridium difficile infection. Am J Physiol Gastrointest Liver Physiol. 2014;306(4):G310-G319.
Thanissery R, McLaren MR, Rivera A, et al. Clostridioides difficile carriage in animals and the associated changes in the host fecal microbiota. Anaerobe. 2020;66:102279.
Weese JS, Armstrong J. Outbreak of Clostridium difficile–associated disease in a small animal veterinary teaching hospital. J Vet Intern Med. 2003;17(6):813-816.
Weese JS, Staempfli HR, Prescott JF, Kruth SA, Greenwood SJ, Weese HE. The roles of Clostridium difficile and enterotoxigenic Clostridium perfringens in diarrhea in dogs. J Vet Intern Med. 2001;15(4):374-378.
Clooten J, Kruth S, Arroyo L, Weese JS. Prevalence and risk factors for Clostridium difficile colonization in dogs and cats hospitalized in an intensive care unit. Vet Microbiol. 2008;129(1-2):209-214.
Sugita K, Yanuma N, Ohno H, et al. Oral faecal microbiota transplantation for the treatment of Clostridium difficile–associated diarrhoea in a dog: a case report. BMC Vet Res. 2019;15(1):11.
Kelly CR, Yen EF, Grinspan AM, et al. Fecal microbiota transplantation is highly effective in real-world practice: initial results from the FMT national registry. Gastroenterology. 2021;160(1):183-192.e3.
McGowan RTS. “Oiling the brain” or “cultivating the gut": impact of diet on anxious behavior in dogs. Purina Companion Animal Nutrition Summit: Pet Nutrition: Beyond Essential; March 31-April 2, 2016; Fort Lauderdale, FL.
Kelley RL, Minikhiem D, Kiely B, et al. Clinical benefits of probiotic canine-derived Bifidobacterium animalis strain AHC7 in dogs with acute idiopathic diarrhea. Vet Ther. 2009;10(3):121-130.
Werner M, Suchodolski JS, Straubinger RK, et al. Effect of amoxicillin-clavulanic acid on clinical scores, intestinal microbiome, and amoxicillin-resistant Escherichia coli in dogs with uncomplicated acute diarrhea. J Vet Intern Med. 2020;34(3):1166-1176.
Shmalberg J, Montalbano C, Morelli G, Buckley GJ. A randomized double blinded placebo-controlled clinical trial of a probiotic or metronidazole for acute canine diarrhea. Front Vet Sci. 2019;6:163.
Minamoto Y, Minamoto T, Isaiah A, et al. Fecal short-chain fatty acid concentrations and dysbiosis in dogs with chronic enteropathy. J Vet Intern Med. 2019;33(4):1608-1618.

Microbiota Modulation Techniques in Dogs

Jan S. Suchodolski, MedVet, DrVetMed, PhD, AGAF, DACVM (Immunology), Gastrointestinal Laboratory at Texas A&M University

Nutrition

References

Clinician's Brief provides relevant diagnostic and treatment information for small animal practitioners. It has been ranked the #1 most essential publication by small animal veterinarians for 9 years.*